INDIGlo Luciferase Detection Reagent Kit

SIZE	SKU	PRICE
10 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-10	—
25 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-25	—
50 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-50	—

SIZE	SKU
10 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-10
25 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-25
50 ml each of-2x concentrated indiglo ldr and dilution buffer	LDR-50

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Specifications
Data
Target Background
Documentation
Overview
Citation
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Product Description and Product Data

INDIGlo Luciferase Detection Reagent from INDIGO Biosciences is a glow type luciferase detection reagent designed for the quantification of firefly and beetle luciferase expression in mammalian cells. The INDIGlo Luciferase Detection Reagent kit is formulated as a 2x-concentrated reagent which contains both the Luciferase Detection Reagent (LDR) and Dilution Buffer and can be used for both homogenous and non-homogenous assay applications. With a signal duration of greater than 2 hours and with a less than 10% fluctuation in signal, the highly sensitive and stable luminescence signal of INDIGlo is ideal for the cost-effective processing of cell culture plates ranging from 96 to 1536-well format.

Features

2X Concentration
Signal Duration >2 Hours
Use for 96- to 1536-Well Formats

Product Specifications

Kit Components		2x-concentrated INDIGlo LDR Dilution Buffer
Shelf Life		6 months
Shipping Requirements		Dry Ice
Storage temperature		-20C

Data

INDIGlo LDR performance from both Homogenous and Non-Homogenous assays. Dose-response analyses of Human AhR (INDIGO #IB06001, 96-well assay) was performed using the reference agonist MeBIO. AhR reporter cells were plated across two white assay plates, MeBIO treatment media were added, and the assay plates incubated for 24 hr. Plates were then processed with luciferase detection reagents in either a.) a homogenous or b.) a non-homogenous assay format. Resulting assay metrics are also shown from using another extended-glo detection reagent (Vendor X).

INDIGlo LDR potency is stable following repeated freeze and thaw cycles. INDIGlo LDR was frozen at -20°C and thawed either 1, 2 or 3 times, then used to quantify luciferase activity from HEK-293:luc cells cultured in a white 96-well assay plate. No loss of reagent potency is observed between these conditions.

Target Background

The glucocorticoid receptor is the receptor that cortisol and other glucocorticoids bind to. The GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response.

INDIGO’s Glucocorticoid Receptor Assay System utilize proprietary mammalian reporter cells engineered to provide constitutive high-level expression of full-length, unmodified human NR3C1 protein, commonly referred to as GR.

INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in GR activity. Luciferase gene expression occurs after ligand-bound GR undergoes nuclear translocation, DNA binding, recruitment, and assembly of the co-activators and accessory factors required to forma functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human androgen receptor.